Rabbit NF kappa B-p65 (Nuclear Factor Kappa B p65) ELISA Kit

Assay Genie

Rabbit NF kappa B-p65 (Nuclear Factor Kappa B p65) ELISA Kit

12

ELISA

46.88-3000 pg/mL

17.5 pg/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit NFkappaB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit NFkappaB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit NFkappaB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Rabbit NFkappaB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.

2°C to 8°C

41116100