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Rabbit NFkappaB-p65(Nuclear Factor Kappa B p65) ELISA Kit

ORB1736725
Biorbyt
ReactivityRabbit
Product group Assays
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Rabbit NFkappaB-p65(Nuclear Factor Kappa B p65) ELISA Kit
  • Delivery Days Customer
    10
  • Application Supplier Note
    standard: 3000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit NFkappaB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit NFkappaB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit NFkappaB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Rabbit NFkappaB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve
  • Assay Detection Range
    46.88-3000 pg/mL
  • Assay Sensitivity
    17.5 pg/mL
  • Certification
    Research Use Only
  • Scientific Description
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit NFkappaB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit NFkappaB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit NFkappaB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Rabbit NFkappaB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.
  • Reactivity
    Rabbit
  • UNSPSC
    41116158

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