Rabbit PGR ELISA Kit

Biorbyt

Rabbit PGR ELISA Kit

10

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit PGR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit PGR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit PGR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Rabbit PGR in the samples is then determined by comparing the OD of the samples to the standard curve

ELISA

ELISA

0.32-20 ng/mL

0.132 ng/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Progesterone Receptor(PGR). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Progesterone Receptor(PGR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Progesterone Receptor(PGR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Progesterone Receptor(PGR) in the samples is then determined by comparing the OD of the samples to the standard curve.

Rabbit

41116158