Gel: 12%SDS-PAGE Lysate: 0.001/0.002/0.005/0.01/0.025 microg Lane 1-5: 0.001/0.002/0.005/0.01/0.025 microg Green fluorescent protein Primary antibody: TA100030 (GFP Antibody) at dilution 1/1000 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 10 seconds