Anti-GFP Purified

EXBIO

Anti-GFP Purified

7

Western blotting: Recommended dilution 0.5-1.5 microg/ml; positive control: transfected cells; negative control: non-transfected cells. Immunocytochemistry: Recommended dilution 1-3 microg/ml.

ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry

Research Use Only

PAb (476)

1 mg/ml

Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (lambda=509 nm) when excited (excitation by Blue or UV light, absorption peak lambda=395 nm). GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chomophore mutations have been created. The Enhanced GFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. yellow fluorescent protein, cyan fluorescent protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.

2°C to 8°C

12352203