Anti-MEK1/2 Purified

EXBIO

Anti-MEK1/2 Purified

7

Western blotting: Recommended dilution 0.5 microg/ml; positive material: RAJI human Burkitt lymphoma cell line, both reducing and non-reducing conditions, reducing conditions are recommended.

Western Blot

Research Use Only

PAb (508)

1 mg/ml

MEK (MAPKK, kinase of mitogen-activated protein kinase) is the medium one of three components of MAP kinase cascade - an important signaling pathway that regulates cell growth and differentiation. Raf (MAPKKK) activates MEK 1 and 2 via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK (MAPK, mitogen-activated protein kinase). This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade. Phosphorylated ERK is capable of translocating to the nucleus, where it regulates gene expression by activating transcription factors.

Human

2°C to 8°C

12352203