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Western Blot analysis of 3T3 cells using Rac1/2/3/CDC42 Polyclonal Antibody
Western Blot analysis of 3T3 cells using Rac1/2/3/CDC42 Polyclonal Antibody
Western Blot analysis of 3T3 cells using Rac1/2/3/CDC42 Polyclonal Antibody

RAC1/RAC2/RAC3/CDC42 Antibody

CSB-PA003908
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDC42
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Overview

  • Supplier
    Cusabio
  • Product Name
    RAC1/RAC2/RAC3/CDC42 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID998
  • Target name
    CDC42
  • Target description
    cell division cycle 42
  • Target synonyms
    CDC42Hs; cell division control protein 42 homolog; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K; G25K GTP-binding protein; growth-regulating protein; GTP binding protein, 25kDa; small GTP binding protein CDC42; TKS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15153
  • Protein Name
    Ras-related C3 botulinum toxin substrate 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDC42 using anti-CDC42 antibody (A00119-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC42 antigen affinity purified polyclonal antibody (Catalog # A00119-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC42 at approximately 21 kDa. The expected band size for CDC42 is at 21 kDa.
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