Western blot All lanes: Cell cycle checkpoint protein RAD1 antibody at 6ug/ml + NIH/3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 32, 28, 8 kDa Observed band size: 32 kDa
RAD1 Antibody
CSB-PA019252ESR1HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRAD1
Overview
- SupplierCusabio
- Product NameRAD1 Antibody
- Delivery Days Customer20
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID5810
- Target nameRAD1
- Target descriptionRAD1 checkpoint DNA exonuclease
- Target synonymscell cycle checkpoint protein Hrad1; cell cycle checkpoint protein RAD1; checkpoint control protein HRAD1; DNA repair exonuclease rad1 homolog; DNA repair exonuclease REC1; exonuclease homolog RAD1; HRAD1; RAD1 checkpoint clamp component; RAD1 homolog; rad1-like DNA damage checkpoint protein; REC1
- HostRabbit
- IsotypeIgG
- Protein IDO60671
- Protein NameCell cycle checkpoint protein RAD1
- Scientific DescriptionComponent of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3\\\-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3\\\->5\\\ double stranded DNA exonuclease activity.
- ReactivityHuman
- Storage Instruction-20°C or -80°C
- UNSPSC12352203