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Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using RAD54L2 Polyclonal Antibody at dilution 1:40
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using RAD54L2 Polyclonal Antibody at dilution 1:40
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using RAD54L2 Polyclonal Antibody at dilution 1:40

RAD54L2 Polyclonal Antibody

E-AB-10576
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    RAD54L2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.6mg/ml
  • Scientific Description
    Adrenergic receptors (ARs) include four general types (a1, a2, b1 and b2) that are found in different target tissues and differ in their affinities and responses to various agonists and antagonists. The coupling of ARs to specific intracellular effectors is mediated through diverse heterotrimeric G proteins. ARs play a critical role in the development of prostate cancer, and transcriptional activity of AR is partly regulated by coregulatory proteins. RAD54L2 (RAD54-like 2), also known as ARIP4 (androgen receptor-interacting protein 4), HSPC325 or SRISNF2L, is a 1,467 amino acid nuclear protein belonging to the SNF2/RAD54 helicase family that consists of one helicase ATP-binding domain and a helicase C-terminal domain. RAD54L2 is a DNA helicase that regulates androgen receptor (AR)-dependent transactivation in a promoter-dependent manner. RAD54L2 is post-translationally sumoylated or phosphorylated upon DNA damage.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAD54L2 using anti-RAD54L2 antibody (A11741). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse Raw264.7 whole cell lysates, Lane 6: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD54L2 antigen affinity purified polyclonal antibody (Catalog # A11741) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAD54L2 at approximately 190 kDa. The expected band size for RAD54L2 is at 163 kDa.
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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA098715(RAD54L2 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)
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