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Western blot analysis of extract from mouse brain tissue and C6 cells using Raf-1 Antibody
Western blot analysis of extract from mouse brain tissue and C6 cells using Raf-1 Antibody
Western blot analysis of extract from mouse brain tissue and C6 cells using Raf-1 Antibody

Raf1 Antibody

Research Use Only
CSB-PA950764
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityRat
TargetRaf1
Price on request
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More than 130 suppliers
ISO certified
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Overview

  • Supplier
    Cusabio
  • Product Name
    Raf1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID24703
  • Target name
    Raf1
  • Target description
    Raf-1 proto-oncogene, serine/threonine kinase
  • Target synonyms
    cRaf; C-RAF; murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV); proto-oncogene c-RAF; RAF proto-oncogene serine/threonine-protein kinase; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-leukemia viral oncogene 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11345
  • Protein Name
    RAF proto-oncogene serine/threonine-protein kinase
  • Scientific Description
    A-Raf, B-Raf and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1). Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497 and Ser499 (2). p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338 and the Src family phosphorylates Tyr341 to induce c-Raf activity (3,4). Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf (5). Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively (6,7). While A-Raf, B-Raf and c-Raf are similar in sequence and function, differential regulation has been observed (8). Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428 and Thr439) and lacks a site equivalent to Tyr341 of c-Raf (8,9). The B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma (10). Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301 and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to Avruch, J. et al. (1994) Trends Biochem. Sci. 19, 279-283. Chong, H. et al. (2001) EMBO J. 20, 3716-3727. King, A.J. et al. (1998) Nature 396, 180-183. Fabian, J.R. et al. (1993) Mol. Cell Biol. 13, 7170-7179.
  • Reactivity
    Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203