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RAGE Antibody

HY-P81086
MedChem Express
TargetAGER
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    RAGE Antibody
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID177
  • Target name
    AGER
  • Target description
    advanced glycosylation end-product specific receptor
  • Target synonyms
    advanced glycosylation end product-specific receptor; RAGE; receptor for advanced glycation end-products variant 20; SCARJ1; sRAGE
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    RAGE Antibody is an unconjugated, approximately 42 kDa, rabbit-derived, anti-RAGE polyclonal antibody. RAGE Antibody can be used for: WB, ELISA, IHC-P, IHC-F, Flow-Cyt, IF expriments in human, mouse, rat, background without labeling.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAGE/AGER using anti-RAGE/AGER antibody (A03438-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAGE/AGER antigen affinity purified polyclonal antibody (Catalog # A03438-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAGE/AGER at approximately 45-58 kDa. The expected band size for RAGE/AGER is at 43 kDa.
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