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Western Blot analysis of 3T3 cells using RAGE Polyclonal Antibody
Western Blot analysis of 3T3 cells using RAGE Polyclonal Antibody
Western Blot analysis of 3T3 cells using RAGE Polyclonal Antibody

AGER Antibody

CSB-PA007810
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAGER
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Overview

  • Supplier
    Cusabio
  • Product Name
    AGER Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID177
  • Target name
    AGER
  • Target description
    advanced glycosylation end-product specific receptor
  • Target synonyms
    advanced glycosylation end product-specific receptor; RAGE; receptor for advanced glycation end-products variant 20; SCARJ1; sRAGE
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15109
  • Protein Name
    Advanced glycosylation end product-specific receptor
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RAGE/AGER using anti-RAGE/AGER antibody (A03438-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAGE/AGER antigen affinity purified polyclonal antibody (Catalog # A03438-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAGE/AGER at approximately 45-58 kDa. The expected band size for RAGE/AGER is at 43 kDa.
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