GPRC5A antibody [HL1864] detects GPRC5A protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
![Boiled and unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A? antibody [HL1864] (GTX637589) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637589/GTX637589_44872_20241227_WB_KO_competitor_ub_watermark_24123021_582.webp "Boiled and unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A? antibody [HL1864] (GTX637589) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637589/GTX637589_T-44823_20241227_WB_TPM_watermark_24123021_621.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A? antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637589/GTX637589_44872_20241227_WB_TPM_watermark_24123021_977.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A? antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637589/GTX637589_T-44823_20241227_WB_B_ub_24123021_993.webp "Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Non-transfected (–) and transfected (+) unboiled MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A? antibody [HL1864] (GTX637589) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637589/GTX637589_44872_20241227_WB_shRNA_competitor_watermark_24123021_590.webp "Non-transfected (–) and transfected (+) unboiled MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A? antibody [HL1864] (GTX637589) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A? antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637589/GTX637589_44872_20250103_WB_KO_watermark_25010819_959.webp "Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A? antibody [HL1864] (GTX637589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A antibody [HL1864] (GTX637589) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637589/GTX637589_44872_20250515_WB_multiple_B_25052202_929.webp "Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A antibody [HL1864] (GTX637589) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![GPRC5A antibody [HL1864] detects GPRC5A protein by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637589/GTX637589_44872_20250604_IHC-P_25061300_904.webp "GPRC5A antibody [HL1864] detects GPRC5A protein by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![GPRC5A antibody [HL1864] detects GPRC5A protein by immunohistochemical analysis. Sample: Paraffin-embedded human tissues. GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637589/GTX637589_44872_20250604_IHC-P_multiple_RPKM_25061300_732.webp "GPRC5A antibody [HL1864] detects GPRC5A protein by immunohistochemical analysis. Sample: Paraffin-embedded human tissues. GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
GPRC5A antibody [HL1864] detects GPRC5A protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: GPRC5A stained by GPRC5A antibody [HL1864] (GTX637589) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GPRC5A antibody [HL1864]
GTX637589
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetGPRC5A
Overview
- SupplierGeneTex
- Product NameGPRC5A antibody [HL1864]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1864
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID9052
- Target nameGPRC5A
- Target descriptionG protein-coupled receptor class C group 5 member A
- Target synonymsGPCR5A, PEIG-1, RAI3, RAIG1, TIG1, retinoic acid-induced protein 3, G-protein coupled receptor family C group 5 member A, TPA induced gene 1, orphan G-protein-coupling receptor PEIG-1, phorbol ester induced gene 1, phorbol ester induced protein-1, retinoic acid induced 3, retinoic acid responsive, retinoic acid-induced gene 1 protein
- HostRabbit
- IsotypeIgG
- Protein IDQ8NFJ5
- Protein NameRetinoic acid-induced protein 3
- Scientific DescriptionThis gene encodes a member of the type 3 G protein-coupling receptor family, characterized by the signature 7-transmembrane domain motif. The encoded protein may be involved in interaction between retinoid acid and G protein signalling pathways. Retinoic acid plays a critical role in development, cellular growth, and differentiation. This gene may play a role in embryonic development and epithelial cell differentiation. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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