FACS analysis of HeLa cells using GTX83725 RANGAP1 antibody [1B4]. Red : Primary antibody Blue : Negative control antibody
![FACS analysis of Jurkat cells using GTX83725 RANGAP1 antibody [1B4]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX83725/GTX83725_152_FACS_w_23061420_567.webp "FACS analysis of Jurkat cells using GTX83725 RANGAP1 antibody [1B4]. Red : Primary antibody Blue : Negative control antibody")
![FACS analysis of HEK293T cells transfected with either RANGAP1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83725 RANGAP1 antibody [1B4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83725/GTX83725_153_FACS_w_23061420_548.webp "FACS analysis of HEK293T cells transfected with either RANGAP1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83725 RANGAP1 antibody [1B4].")
![IHC-P analysis of human ovary adenocarcinoma tissue using GTX83725 RANGAP1 antibody [1B4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83725/GTX83725_1766_IHC-P_w_23061420_637.webp "IHC-P analysis of human ovary adenocarcinoma tissue using GTX83725 RANGAP1 antibody [1B4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of human liver tissue using GTX83725 RANGAP1 antibody [1B4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83725/GTX83725_1767_IHC-P_w_23061420_342.webp "IHC-P analysis of human liver tissue using GTX83725 RANGAP1 antibody [1B4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of human prostate tissue using GTX83725 RANGAP1 antibody [1B4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83725/GTX83725_1768_IHC-P_w_23061420_979.webp "IHC-P analysis of human prostate tissue using GTX83725 RANGAP1 antibody [1B4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![WB analysis of NIH3T3 cell lysate using GTX83725 RANGAP1 antibody [1B4]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX83725/GTX83725_3447_WB_w_23061420_130.webp "WB analysis of NIH3T3 cell lysate using GTX83725 RANGAP1 antibody [1B4]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX83725 RANGAP1 antibody [1B4]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX83725/GTX83725_3735_WB_w_23061420_142.webp "WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX83725 RANGAP1 antibody [1B4]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of HEK293T cells transfected with RANGAP1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83725 RANGAP1 antibody [1B4]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83725/GTX83725_3853_WB_w_23061420_834.webp "WB analysis of HEK293T cells transfected with RANGAP1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83725 RANGAP1 antibody [1B4]. Loading : 5 ug per lane")
![WB analysis of various cell lines using GTX83725 RANGAP1 antibody [1B4]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83725/GTX83725_3855_WB_w_23061420_353.webp "WB analysis of various cell lines using GTX83725 RANGAP1 antibody [1B4]. Loading : 35 ug per lane")
FACS analysis of HeLa cells using GTX83725 RANGAP1 antibody [1B4]. Red : Primary antibody Blue : Negative control antibody
RANGAP1 antibody [1B4]
GTX83725
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRANGAP1
Overview
- SupplierGeneTex
- Product NameRANGAP1 antibody [1B4] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:2000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1B4
- Concentration0.81 mg/ml
- ConjugateUnconjugated
- Gene ID5905
- Target nameRANGAP1
- Target descriptionRan GTPase activating protein 1
- Target synonymsFug1; ran GTPase-activating protein 1; RANGAP; SD; segregation distorter homolog
- HostMouse
- IsotypeIgG2a
- Protein IDP46060
- Protein NameRan GTPase-activating protein 1
- Scientific DescriptionGTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
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