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ProductsBack/RANGAP1 Mouse Monoclonal Antibody [Clone ID: OTI1B4]
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human RANGAP1 plasmid (RC204223, hRANGAP1), mouse RANGAP1 plasmid (MR209194, mRANGAP1) using anti-RANGAP1 antibody TA501265 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human RANGAP1 plasmid (RC204223, hRANGAP1), mouse RANGAP1 plasmid (MR209194, mRANGAP1) using anti-RANGAP1 antibody TA501265 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human RANGAP1 plasmid (RC204223, hRANGAP1), mouse RANGAP1 plasmid (MR209194, mRANGAP1) using anti-RANGAP1 antibody TA501265 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

RANGAP1 Mouse Monoclonal Antibody [Clone ID: OTI1B4]

CF501265
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetRANGAP1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    RANGAP1 Mouse Monoclonal Antibody [Clone ID: OTI1B4]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1B4
  • Gene ID5905
  • Target name
    RANGAP1
  • Target description
    Ran GTPase activating protein 1
  • Target synonyms
    Fug1, RANGAP, SD, ran GTPase-activating protein 1, segregation distorter homolog
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP46060
  • Protein Name
    Ran GTPase-activating protein 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) RANGAP1 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4)
  • Reactivity
    Canine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RANGAP1 using anti-RANGAP1 antibody (A02771-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human Hacat whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: human HL-60 whole cell lysates, Lane 8: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANGAP1 antigen affinity purified polyclonal antibody (Catalog # A02771-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANGAP1 at approximately 70 kDa. The expected band size for RANGAP1 is at 70 kDa.
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