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Immunofluorescence analysis of MCF-7 cells using RANGAP1 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using RANGAP1 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using RANGAP1 Polyclonal Antibody

RANGAP1 Polyclonal Antibody

E-AB-64572
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    RANGAP1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RANGAP1 using anti-RANGAP1 antibody (A02771-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human Hacat whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: human HL-60 whole cell lysates, Lane 8: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANGAP1 antigen affinity purified polyclonal antibody (Catalog # A02771-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANGAP1 at approximately 70 kDa. The expected band size for RANGAP1 is at 70 kDa.
Product group Antibodies
Boster Bio
Anti-RANGAP1 Antibody Picoband(r)A02771-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRANGAP1
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Product group Antibodies
RayBiotech
Anti-RANGAP1 Antibody144-05381
ApplicationsWestern Blot, ImmunoHistoChemistry
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Product group Antibodies
Bioss
RANGAP1 Recombinant AntibodyBSM-61906R
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetRANGAP1
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Product group Antibodies
Cusabio
RANGAP1 AntibodyCSB-PA019318GA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRANGAP1
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RanGAP1 AntibodyCAB5381
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Product group Antibodies
Atlas Antibodies
Anti-RANGAP1 AntibodyHPA050110
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetRANGAP1
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Product group Antibodies
Everest Biotech
Goat anti-RANGAP1 AntibodyEB06253
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TargetRANGAP1
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MedChem Express
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Immunohistochemical analysis of paraffin-embedded human Trachea, using RanGAP1(GTX106338) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Product group Antibodies
GeneTex
RanGAP1 antibody [N1], N-termGTX106338
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
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TargetRANGAP1
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