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ProductsBack/RANK antibody [12G33]

RANK antibody [12G33]

GTX53423
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse
TargetTnfrsf11a
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Overview

  • Supplier
    GeneTex
  • Product Name
    RANK antibody [12G33]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:250 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    12G33
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID21934
  • Target name
    Tnfrsf11a
  • Target description
    tumor necrosis factor receptor superfamily, member 11a, NFKB activator
  • Target synonyms
    Ly109, ODFR, OFE, Rank, TRANCE-R, mRANK, tumor necrosis factor receptor superfamily member 11A, osteoclast differentiation factor receptor, receptor activator of NF-KB, receptor activator of NF-kappaB
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDO35305
  • Protein Name
    Tumor necrosis factor receptor superfamily member 11A
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Tnfrsf11a using anti-Tnfrsf11a antibody (A01064-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates, Lane 4: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tnfrsf11a antigen affinity purified polyclonal antibody (Catalog # A01064-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tnfrsf11a at approximately 80KD. The expected band size for Tnfrsf11a is at 66KD.
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