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RAP1A Antibody (clone 5F8)

LS-C169179
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetRAP1A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RAP1A Antibody (clone 5F8)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), Flo (1:200 - 1:400), WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5F8
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5906
  • Target name
    RAP1A
  • Target description
    RAP1A, member of RAS oncogene family
  • Target synonyms
    C21KG; G-22K; GTP-binding protein smg p21A; KREV1; KREV-1; RAP1; Ras-related protein Krev-1; ras-related protein Rap-1A; SMGP21
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAP1A using anti-RAP1A antibody (PB9816). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Liver Tissue Lysate, Lane 3: Rat Kidney Tissue Lysate, Lane 4: MCF-7 Whole Cell Lysate, Lane 5: SW620 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP1A antigen affinity purified polyclonal antibody (Catalog # PB9816) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAP1A at approximately 21KD. The expected band size for RAP1A is at 21KD.
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Immunofluorescence staining of Hela cells with CSB-PA019321LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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