More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/RAP1A Antibody
Immunofluorescence staining of Hela cells with CSB-PA019321LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cells with CSB-PA019321LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cells with CSB-PA019321LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

RAP1A Antibody

CSB-PA019321LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRAP1A
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Cusabio
  • Product Name
    RAP1A Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5906
  • Target name
    RAP1A
  • Target description
    RAP1A, member of RAS oncogene family
  • Target synonyms
    C21KG; G-22K; GTP-binding protein smg p21A; KREV1; KREV-1; RAP1; Ras-related protein Krev-1; ras-related protein Rap-1A; SMGP21
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62834
  • Protein Name
    Ras-related protein Rap-1A
  • Scientific Description
    Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes. Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

Related products

Formalin-fixed and paraffin embedded human lung cancer tissue labeled with Anti-RAP1A Polyclonal Antibody, Unconjugated (bs-1504R) at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Product group Antibodies
Bioss Antibodies
RAP1A Polyclonal AntibodyBS-1504R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetRAP1A
  • SizePrice
Product group Antibodies
Biomatik
RAP1A Polyclonal AntibodyCAC13180
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetRAP1A
  • SizePrice
Product group Antibodies
Assay Genie
Rap1A AntibodyCAB0975
TargetRAP1A
  • SizePrice
Anti-RAP1 Antibody
Product group Antibodies
Antibodies.com
Anti-RAP1 AntibodyA101320
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
RayBiotech
Anti-RAP1A Antibody144-00975
ApplicationsWestern Blot
TargetRAP1A
  • SizePrice
Product group Antibodies
GeneTex
RAP1 antibodyGTX102770
ApplicationsWestern Blot
TargetRAP1A
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
RAP1A AntibodyLS-C403843
ApplicationsELISA, ImmunoHistoChemistry
TargetRAP1A
  • SizePrice
Figure 1. Western blot analysis of RAP1A using anti-RAP1A antibody (PB9816). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Liver Tissue Lysate, Lane 3: Rat Kidney Tissue Lysate, Lane 4: MCF-7 Whole Cell Lysate, Lane 5: SW620 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP1A antigen affinity purified polyclonal antibody (Catalog # PB9816) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAP1A at approximately 21KD. The expected band size for RAP1A is at 21KD.
Product group Antibodies
Boster Bio
Anti-RAP1A Antibody Picoband(r)PB9816-CARRIER-FREE
ApplicationsWestern Blot
TargetRAP1A
  • SizePrice