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Western Blot Positive WB detected in: HL60 whole cell lysate All lanes: RARS antibody at 3.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76, 68 kDa Observed band size: 76 kDa
Western Blot Positive WB detected in: HL60 whole cell lysate All lanes: RARS antibody at 3.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76, 68 kDa Observed band size: 76 kDa
Western Blot Positive WB detected in: HL60 whole cell lysate All lanes: RARS antibody at 3.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76, 68 kDa Observed band size: 76 kDa

RARS Antibody

CSB-PA019344LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRARS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RARS Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5917
  • Target name
    RARS1
  • Target description
    arginyl-tRNA synthetase 1
  • Target synonyms
    arginine tRNA ligase 1, cytoplasmic; arginine--tRNA ligase, cytoplasmic; arginyl-tRNA synthetase, cytoplasmic; ArgRS; DALRD1; HLD9; RARS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54136
  • Protein Name
    Arginine--tRNA ligase, cytoplasmic
  • Scientific Description
    Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RARS/RARS1 using anti-RARS/RARS1 antibody (A01418-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RARS/RARS1 antigen affinity purified polyclonal antibody (Catalog # A01418-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RARS/RARS1 at approximately 70 kDa. The expected band size for RARS/RARS1 is at 75 kDa.
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