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Western Blot Positive WB detected in: Hela whole cell lysate, Mouse brain tissue All lanes: RASA1 antibody at 3.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 117, 101, 59, 102 kDa Observed band size: 117 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Mouse brain tissue All lanes: RASA1 antibody at 3.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 117, 101, 59, 102 kDa Observed band size: 117 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Mouse brain tissue All lanes: RASA1 antibody at 3.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 117, 101, 59, 102 kDa Observed band size: 117 kDa

RASA1 Antibody

CSB-PA019346LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetRASA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RASA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5921
  • Target name
    RASA1
  • Target description
    RAS p21 protein activator 1
  • Target synonyms
    CM-AVM, CMAVM, CMAVM1, GAP, PKWS, RASA, RASGAP, p120, p120GAP, p120RASGAP, ras GTPase-activating protein 1, RAS p21 protein activator (GTPase activating protein) 1, p120 RAS GTPase activating protein, triphosphatase-activating protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20936
  • Protein Name
    Ras GTPase-activating protein 1
  • Scientific Description
    Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21; this stimulation may be further increased in the presence of NCK1.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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ICC/IF analysis of Hela cells using GTX22922 RASA1 antibody [B4F8]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC
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Figure 1. Western blot analysis of RASA1 using anti-RASA1 antibody (PB9796). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: MCF-7 Whole Cell Lysate, Lane 3: SMMC Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASA1 antigen affinity purified polyclonal antibody (Catalog # PB9796) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASA1 at approximately 100 kDa. The expected band size for RASA1 is at 100 kDa.
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