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Gel: 6%SDS-PAGE Lysate: 40 microg Lane: Mouse brain tissue Primary antibody: TA364958 (RASAL2 Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds
Gel: 6%SDS-PAGE Lysate: 40 microg Lane: Mouse brain tissue Primary antibody: TA364958 (RASAL2 Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds
Gel: 6%SDS-PAGE Lysate: 40 microg Lane: Mouse brain tissue Primary antibody: TA364958 (RASAL2 Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds

RASAL2 Rabbit Polyclonal Antibody

TA364958
OriGene
ApplicationsWestern Blot
DownloadMSDS
Product group Antibodies
TargetRASAL2
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    RASAL2 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID9462
  • Target name
    RASAL2
  • Target description
    RAS protein activator like 2
  • Target synonyms
    NGAP; ras GTPase-activating protein nGAP; Ras protein activator like 1; RASAL2/ACVR1 fusion
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UJF2
  • Protein Name
    Ras GTPase-activating protein nGAP
  • Scientific Description
    RASAL2 rabbit polyclonal antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RASAL2 using anti-RASAL2 antibody (A07356-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASAL2 antigen affinity purified polyclonal antibody (Catalog # A07356-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASAL2 at approximately 150 kDa. The expected band size for RASAL2 is at 129 kDa.
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Lane 1: Human Hela cell lysates; Lane 2: Human MDA-MB-231 cell lysates; Lane 3: Human MKN45 cell lysates; Lane 4: Human HepG2 celll lysates probed with RASAL2 Polyclonal Antibody, Unconjugated (bs-21160R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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