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WB analysis of various sample lysates using GTX64637 RASGRP1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64637 RASGRP1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64637 RASGRP1 antibody. Dilution : 1:1000 Loading : 25microg per lane

RASGRP1 antibody

GTX64637
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetRASGRP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    RASGRP1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10125
  • Target name
    RASGRP1
  • Target description
    RAS guanyl releasing protein 1
  • Target synonyms
    calcium and DAG-regulated guanine nucleotide exchange factor II; calcium- and diacylglycerol-regulated guanine nucleotide exchange factor II; CALDAG-GEFI; CALDAG-GEFII; guanine nucleotide exchange factor, calcium- and DAG-regulated, Rap1A; IMD64; ras activator RasGRP; RAS guanyl nucleotide-releasing protein 1; RAS guanyl releasing protein 1 (calcium and DAG-regulated); RAS guanyl-releasing protein 1; RASGRP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95267
  • Protein Name
    RAS guanyl-releasing protein 1
  • Scientific Description
    This gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation. Alternatively spliced transcript variants encoding different isoforms have been identified. Altered expression of the different isoforms of this protein may be a cause of susceptibility to systemic lupus erythematosus (SLE). [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RASGRP1 using anti-RASGRP1 antibody (A03004-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASGRP1 antigen affinity purified polyclonal antibody (Catalog # A03004-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASGRP1 at approximately 100 kDa. The expected band size for RASGRP1 is at 90 kDa.
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