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Western Blot Positive WB detected in: Raji whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate All lanes: RASSF2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38, 18 kDa Observed band size: 38 kDa
Western Blot Positive WB detected in: Raji whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate All lanes: RASSF2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38, 18 kDa Observed band size: 38 kDa
Western Blot Positive WB detected in: Raji whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate All lanes: RASSF2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38, 18 kDa Observed band size: 38 kDa

RASSF2 Antibody

CSB-PA019374LA01HU
Cusabio
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetRASSF2
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Overview

  • Supplier
    Cusabio
  • Product Name
    RASSF2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9770
  • Target name
    RASSF2
  • Target description
    Ras association domain family member 2
  • Target synonyms
    CENP-34, RASFADIN, ras association domain-containing protein 2, Ras association (RalGDS/AF-6) domain family 2, Ras association (RalGDS/AF-6) domain family member 2, centromere protein 34
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50749
  • Protein Name
    Ras association domain-containing protein 2
  • Scientific Description
    Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. Stabilizes STK3/MST2 by protecting it from proteasomal degradation.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RASSF2 using anti-RASSF2 antibody (A04384-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASSF2 antigen affinity purified polyclonal antibody (Catalog # A04384-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASSF2 at approximately 38 kDa. The expected band size for RASSF2 is at 38 kDa.
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