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ProductsBack/Rat Anti-Human IL-4-UNLB

Rat Anti-Human IL-4-UNLB

10117-01
SouthernBiotech
ApplicationsFlow Cytometry, ELISA, ELISpot Assay, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman
TargetIL4
0.5 mg
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Overview

  • Supplier
    SouthernBiotech
  • Product Name
    Rat Anti-Human IL-4-UNLB
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry, ELISA, ELISpot Assay, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MP4-25D2
  • Conjugate
    Unconjugated
  • Gene ID3565
  • Target name
    IL4
  • Target description
    interleukin 4
  • Target synonyms
    BCGF-1, BCGF1, BSF-1, BSF1, IL-4, interleukin-4, B cell growth factor 1, B_cell stimulatory factor 1, binetrakin, lymphocyte stimulatory factor 1, pitrakinra
  • Host
    Rat
  • Isotype
    IgG1
  • Protein IDP05112
  • Protein Name
    Interleukin-4
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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IF(ICC) staining with Interleukin-4 (3F4) Monoclonal Antibody (bsm-54002R) at 1:100 in D3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
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Figure 1. Western blot analysis of IL4 using anti-IL4 antibody (A00230). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CCRF-CEM whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL4 antigen affinity purified polyclonal antibody (Catalog # A00230) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL4 at approximately 20KD. The expected band size for IL4 is at 17KD.
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