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BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD11b-PE (SB Cat. No. 1561-09) and Rat Anti-Mouse Ly-6G/Ly-6C-FITC (SB Cat. No. 1900-02).
BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD11b-PE (SB Cat. No. 1561-09) and Rat Anti-Mouse Ly-6G/Ly-6C-FITC (SB Cat. No. 1900-02).
BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD11b-PE (SB Cat. No. 1561-09) and Rat Anti-Mouse Ly-6G/Ly-6C-FITC (SB Cat. No. 1900-02).

Rat Anti-Mouse CD11b-PE

1561-09
SouthernBiotech
ApplicationsFlow Cytometry
Product group Antibodies
TargetITGAM
Price on request
0.1 mg
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Overview

  • Supplier
    SouthernBiotech
  • Product Name
    Rat Anti-Mouse CD11b-PE
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    M1/70
  • Conjugate
    RPE
  • Gene ID3684
  • Target name
    ITGAM
  • Target description
    integrin subunit alpha M
  • Target synonyms
    CD11B, CR3A, MAC-1, MAC1A, MO1A, SLEB6, integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, antigen CD11b (p170), cell surface glycoprotein MAC-1 subunit alpha, complement component 3 receptor 3 subunit, integrin, alpha M (complement component 3 receptor 3 subunit), leukocyte adhesion receptor MO1, macrophage antigen alpha polypeptide, macrophage-1 antigen alpha subunit, neutrophil adherence receptor alpha-M subunit
  • Host
    Rat
  • Isotype
    IgG2b
  • Protein IDP05555
  • Protein Name
    Integrin alpha-M
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD11b using anti-CD11b antibody (A00144-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11b antigen affinity purified polyclonal antibody (Catalog # A00144-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11b at approximately 170KD. The expected band size for CD11b is at 127KD.
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