Rat BAK1(Bcl2 Antagonist/Killer 1) ELISA Kit

Biorbyt

Rat BAK1(Bcl2 Antagonist/Killer 1) ELISA Kit

10

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BAK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BAK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BAK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Rat BAK1 in the samples is then determined by comparing the OD of the samples to the standard curve

0.16-10 ng/mL

0.052 ng/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Antagonist/Killer 1(BAK1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bcl2 Antagonist/Killer 1(BAK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Antagonist/Killer 1(BAK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bcl2 Antagonist/Killer 1(BAK1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat

41116158