Rat Coagulation factor III/Tissue Factor protein, His tag

GeneTex

Rat Coagulation factor III/Tissue Factor protein (active)

9

TF (Tissue factor) also known for platelet tissue factor, factor III, thromboplastin, or CD142, is a cell surface glyprotein present in subendothelial tissue and leukocytes. TF is necessary for the initiation of thrombin formation from the zymogen prothrombin. It has been proven that EGF can bind with the TF on the hemangioendotheliocytes. Thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TF and recombinant rat EGF. Briefly, TF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 microl TF were then transferred to EGF-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-TF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 microl stop solution to the wells and read at 450nm immediately. The binding activity of TF and EGF was in a dose dependent manner.

Functional Assay

Research Use Only

Unconjugated

Tissue factor

an initiation factor for blood coagulation; plays a critical role in hepatic ischemic reperfusion injury [RGD, Feb 2006]

-20°C or -80°C,2°C to 8°C

12352202