Rat OXR1(Oxidation resistance protein 1) ELISA Kit

Biorbyt

Rat OXR1(Oxidation resistance protein 1) ELISA Kit

10

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat OXR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OXR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat OXR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Rat OXR1 in the samples is then determined by comparing the OD of the samples to the standard curve

0.16-10 ng/mL

0.055 ng/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oxidation resistance protein 1(OXR1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Oxidation resistance protein 1(OXR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oxidation resistance protein 1(OXR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Oxidation resistance protein 1(OXR1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat

41116158