Rat PAP(Plasmin-Antiplasmin Complex) ELISA Kit

Biorbyt

Rat PAP(Plasmin-Antiplasmin Complex) ELISA Kit

10

standard: 500 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Rat PAP in the samples is then determined by comparing the OD of the samples to the standard curve

7.81-500 ng/mL

4.82 ng/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plasmin-Antiplasmin Complex(PAP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Plasmin-Antiplasmin Complex(PAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Plasmin-Antiplasmin Complex(PAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Plasmin-Antiplasmin Complex(PAP) in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat

41116158