Rat Phospho-Akt (S473) ELISA Kit

Biorbyt

Rat Phospho-Akt (S473) ELISA Kit

10

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Phospho-Akt. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Phospho-Akt. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Phospho-Akt, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Rat Phospho-Akt in the samples is then determined by comparing the OD of the samples to the standard curve

15.63-1000 pg/mL

6.4 pg/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Phospho-Akt. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Phospho-Akt. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Phospho-Akt, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Rat Phospho-Akt in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat

41116158