Deglycosylation of GTX65638-pro rat progranulin (DDDDK tagged) protein. To examine the deglycosylation of rat Progranulin (DDDDDK tagged), 1 μg of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100oC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37oC. Separation of reaction products is visualized by immunoblotting using anti-DDDDDK-HRP antibody.
Deglycosylation of GTX65638-pro rat progranulin (DDDDK tagged) protein. To examine the deglycosylation of rat Progranulin (DDDDDK tagged), 1 μg of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100oC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37oC. Separation of reaction products is visualized by immunoblotting using anti-DDDDDK-HRP antibody.
Rat Progranulins protein, DDDDK tag
GTX65638-PRO
Product group Proteins / Signaling Molecules
Overview
- SupplierGeneTex
- Product NameRat Progranulins protein, DDDDK tag
- Delivery Days Customer9
- CertificationResearch Use Only
- ConjugateUnconjugated
- Gene ID29143
- Target nameGrn
- Target descriptiongranulin precursor
- Target synonymsPEPI, PGRN, progranulin, acrogranin, epithelin, granulins, proepithelin, prostate cancer cell-derived growth factor
- Storage Instruction-20°C
- UNSPSC41116120
- SpeciesRat