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Deglycosylation of GTX65638-pro rat progranulin (DDDDK tagged) protein. To examine the deglycosylation of rat Progranulin (DDDDDK tagged), 1 microg of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100oC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37oC. Separation of reaction products is visualized by immunoblotting using anti-DDDDDK-HRP antibody.
Deglycosylation of GTX65638-pro rat progranulin (DDDDK tagged) protein. To examine the deglycosylation of rat Progranulin (DDDDDK tagged), 1 microg of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100oC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37oC. Separation of reaction products is visualized by immunoblotting using anti-DDDDDK-HRP antibody.
Deglycosylation of GTX65638-pro rat progranulin (DDDDK tagged) protein. To examine the deglycosylation of rat Progranulin (DDDDDK tagged), 1 microg of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100oC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37oC. Separation of reaction products is visualized by immunoblotting using anti-DDDDDK-HRP antibody.

Rat Progranulins protein

Research Use Only
GTX65638-PRO
GeneTex
Product group Proteins / Signaling Molecules
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Overview

  • Supplier
    GeneTex
  • Product Name
    Rat Progranulins protein
  • Delivery Days Customer
    9
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Storage Instruction
    -20°C
  • UNSPSC
    12352202