Rat TGF beta 2 protein, His tag

GeneTex

Rat TGF beta 2 protein (active)

9

Transforming growth factor-beta 2 (TGF-beta2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development (alternative names: Glioblastoma-derived T-cell suppressor factor, G-TSF, BSC-1 cell growth inhibitor, Polyergin, Cetermin). It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. Besides, Vitronectin (VTN) has been identified as an interactor of TGF-beta2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TGF-beta2 and recombinant rat VTN. Briefly, TGF-beta2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 microl were then transferred to VTN-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-TGF-beta2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 microl stop solution to the wells and read at 450nm immediately. The binding activity of TGF-beta2 and VTN was in a dose dependent manner.

Functional Assay

Research Use Only

Unconjugated

Transforming growth factor beta-2

binds the transforming growth factor-beta receptor; plays a role in regulation of cell growth and proliferation; may be involved in mesenchymal-epithelial cell interactions during development [RGD, Feb 2006]

-20°C or -80°C,2°C to 8°C

12352202