Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rb antibody [HL2465] (GTX638814) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rb antibody [HL2465] (GTX638814) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638814/GTX638814_45166_20230915_WB_23091901_835.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rb antibody [HL2465] (GTX638814) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Rb antibody [HL2465] detects Rb protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rb stained by Rb antibody [HL2465] (GTX638814) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638814/GTX638814_45166_20240112_ICC_IF_24013018_559.webp "Rb antibody [HL2465] detects Rb protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rb stained by Rb antibody [HL2465] (GTX638814) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rb antibody [HL2465] (GTX638814) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Rb antibody [HL2465]
GTX638814
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetRB1
Overview
- SupplierGeneTex
- Product NameRb antibody [HL2465]
- Delivery Days Customer9
- Application Supplier Note*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2465
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5925
- Target nameRB1
- Target descriptionRB transcriptional corepressor 1
- Target synonymsOSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110, retinoblastoma-associated protein, prepro-retinoblastoma-associated protein, protein phosphatase 1, regulatory subunit 130, retinoblastoma 1, retinoblastoma suspectibility protein
- HostRabbit
- IsotypeIgG
- Protein IDP06400
- Protein NameRetinoblastoma-associated protein
- Scientific DescriptionThe protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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![Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rb (phospho Ser780) antibody [HL3285] (GTX640939) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640939/GTX640939_T-45530_20240913_WB_treatment_CIP_24091901_348.webp)
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