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Western Blot Positive WB detected in: NIH/3T3 whole cell lysate All lanes: RBFOX2 antibody at 6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 41, 38, 40, 48, 44 kDa Observed band size: 42 kDa
Western Blot Positive WB detected in: NIH/3T3 whole cell lysate All lanes: RBFOX2 antibody at 6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 41, 38, 40, 48, 44 kDa Observed band size: 42 kDa
Western Blot Positive WB detected in: NIH/3T3 whole cell lysate All lanes: RBFOX2 antibody at 6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 41, 38, 40, 48, 44 kDa Observed band size: 42 kDa

RBFOX2 Antibody

CSB-PA019439LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRBFOX2
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Overview

  • Supplier
    Cusabio
  • Product Name
    RBFOX2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23543
  • Target name
    RBFOX2
  • Target description
    RNA binding fox-1 homolog 2
  • Target synonyms
    dJ106I20.3; fox-1 homolog B; fox-1 homologue; FOX2; Fox-2; fxh; hexaribonucleotide-binding protein 2; HNRBP2; HRNBP2; RBM9; repressor of tamoxifen transcriptional activity; RNA binding protein fox-1 homolog 2; RNA binding protein, fox-1 homolog 2; RNA-binding motif protein 9; RTA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43251
  • Protein Name
    RNA binding protein fox-1 homolog 2
  • Scientific Description
    RNA-binding protein that regulates alternative splicing events by binding to 5-UGCAUGU-3 elements. Prevents binding of U2AF2 to the 3-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis (By similarity). RNA-binding protein that seems to act as a coregulatory factor of ER-alpha.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. IHC analysis of RBFOX2 using anti-RBFOX2 antibody (A05389-1). RBFOX2 was detected in paraffin-embedded section of human melanoma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-RBFOX2 Antibody (A05389-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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