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RBM12 Antibody

LS-C830641
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetRBM12
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RBM12 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:40 - 1:200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10137
  • Target name
    RBM12
  • Target description
    RNA binding motif protein 12
  • Target synonyms
    HRIHFB2091, SCZD19, SWAN, RNA-binding protein 12, SH3/WW domain anchor protein in the nucleus
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of SWAN/RBM12 using anti-SWAN/RBM12 antibody (A13929). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SWAN/RBM12 antigen affinity purified polyclonal antibody (Catalog # A13929) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SWAN/RBM12 at approximately 97 kDa. The expected band size for SWAN/RBM12 is at 97 kDa.
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