Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RBM15(GTX109899) antibody at 1:200 dilution.
![HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RBM15 antibody [N1N3] (GTX109899) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109899/GTX109899_43852_20200221_WB_Fraction_w_23060500_990.webp "HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RBM15 antibody [N1N3] (GTX109899) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![RBM15 antibody [N1N3] detects RBM15 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. RBM15 stained by RBM15 antibody [N1N3] (GTX109899) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX109899/GTX109899_44007_20200828_IHC-P_M_w_23060500_616.webp "RBM15 antibody [N1N3] detects RBM15 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. RBM15 stained by RBM15 antibody [N1N3] (GTX109899) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RBM15(GTX109899) antibody at 1:200 dilution.
RBM15 antibody [N1N3]
GTX109899
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetRBM15
Overview
- SupplierGeneTex
- Product NameRBM15 antibody [N1N3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.28 mg/ml
- ConjugateUnconjugated
- Gene ID64783
- Target nameRBM15
- Target descriptionRNA binding motif protein 15
- Target synonymsOTT, OTT1, SPEN, RNA-binding protein 15, one twenty two protein, one twenty-two, one-twenty two protein 1, putative RNA-binding protein 15
- HostRabbit
- IsotypeIgG
- Protein IDQ96T37
- Protein NameRNA-binding protein 15
- Scientific DescriptionMembers of the SPEN (Split-end) family of proteins, including RBM15, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 [PubMed 16129689]).[supplied by OMIM]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Sundararaman B, Zhan L, Blue SM, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016,61(6):903-13. doi: 10.1016/j.molcel.2016.02.012Read this paper
Datasheet
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