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RBM28 Antibody

CSB-PA019419GA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRBM28
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Overview

  • Supplier
    Cusabio
  • Product Name
    RBM28 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55131
  • Target name
    RBM28
  • Target description
    RNA binding motif protein 28
  • Target synonyms
    ANES, NOP4, RNA-binding protein 28, 2810480G15Rik
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NW13
  • Protein Name
    RNA-binding protein 28
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RBM28 using anti-RBM28 antibody (A09394-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM28 antigen affinity purified polyclonal antibody (Catalog # A09394-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBM28 at approximately 100 kDa. The expected band size for RBM28 is at 86 kDa.
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