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Western Blot Positive WB detected in: NIH/3T3 whole cell lysate All lanes: RBMS1 antibody at 3.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 5 kDa Observed band size: 45 kDa
Western Blot Positive WB detected in: NIH/3T3 whole cell lysate All lanes: RBMS1 antibody at 3.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 5 kDa Observed band size: 45 kDa
Western Blot Positive WB detected in: NIH/3T3 whole cell lysate All lanes: RBMS1 antibody at 3.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 5 kDa Observed band size: 45 kDa

RBMS1 Antibody

CSB-PA019440LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetRBMS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RBMS1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5937
  • Target name
    RBMS1
  • Target description
    RNA binding motif single stranded interacting protein 1
  • Target synonyms
    C2orf12; cervical cancer oncogene 4; c-myc gene single strand binding protein 2; HCC-4; MSSP; MSSP-1; MSSP-2; MSSP-3; RNA-binding motif, single-stranded-interacting protein 1; SCR2; single-stranded DNA-binding protein MSSP-1; suppressor of cdc 2 (cdc13) with RNA binding motif 2; suppressor of CDC2 with RNA-binding motif 2; YC1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29558
  • Protein Name
    RNA-binding motif, single-stranded-interacting protein 1
  • Scientific Description
    Single-stranded DNA binding protein that interacts with the region upstream of the MYC gene. Binds specifically to the DNA sequence motif 5-[AT]CT[AT][AT]T-3. Probably has a role in DNA replication.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RBMS1 using anti-RBMS1 antibody (A08752-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBMS1 antigen affinity purified polyclonal antibody (Catalog # A08752-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBMS1 at approximately 50 kDa. The expected band size for RBMS1 is at 45 kDa.
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