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RBX1 Antibody (YA1124, Rabbit)

HY-P81379
MedChem Express
TargetRBX1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    RBX1 Antibody (YA1124, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID9978
  • Target name
    RBX1
  • Target description
    ring-box 1
  • Target synonyms
    BA554C12.1, RNF75, ROC1, E3 ubiquitin-protein ligase RBX1, E3 ubiquitin-protein transferase RBX1, RING finger protein 75, RING-box protein 1, ZYP protein, regulator of cullins 1, ring-box 1, E3 ubiquitin protein ligase
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    RBX1 Antibody (YA1124) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1124), targeting RBX1. RBX1 Antibody (YA1124) can be used for IHC-P experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ROC1 using anti-ROC1 antibody (PB9798). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: Rat Testis Tissue Lysate, Lane 2: Rat Brain Tissue Lysate, Lane 3: Mouse Brain Tissue Lysate, Lane 4: Mouse Spleen Tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROC1 antigen affinity purified polyclonal antibody (Catalog # PB9798) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROC1 at approximately 15 kDa. The expected band size for ROC1 is at 15 kDa.
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