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Western blot analysis of extracts of various cell lines using RCOR1 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using RCOR1 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using RCOR1 Polyclonal Antibody at dilution of 1:3000.

RCOR1 Polyclonal Antibody

E-AB-64348
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    RCOR1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a protein that is well-conserved, downregulated at birth, and with a specific role in determining neural cell differentiation. The encoded protein binds to the C-terminal domain of REST (repressor element-1 silencing transcription factor).
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CoREST/RCOR1 using anti-CoREST/RCOR1 antibody (A02799-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CoREST/RCOR1 antigen affinity purified polyclonal antibody (Catalog # A02799-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CoREST/RCOR1 at approximately 65 kDa. The expected band size for CoREST/RCOR1 is at 53 kDa.
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ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetRCOR1
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Product group Antibodies
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TargetRCOR1
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COREST Recombinant Antibody, AbBy Fluor-405 ConjugatedBSM-61897R-BF405
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ReactivityHuman, Mouse, Rat
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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA019515HA01HU at dilution of 1:100
Product group Antibodies
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RCOR1 AntibodyCSB-PA019515HA01HU
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Product group Antibodies
Atlas Antibodies
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WB analysis of various sample lysates using GTX66126 CoREST antibody. Dilution : 1:3000 Loading : 25microg per lane
Product group Antibodies
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