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ProductsBack/Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Polyclonal Antibody

Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Polyclonal Antibody

CAU26944
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityRat
TargetTnfsf11
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Overview

  • Supplier
    Biomatik
  • Product Name
    Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID117516
  • Target name
    Tnfsf11
  • Target description
    TNF superfamily member 11
  • Target synonyms
    ODF, OPGL, RANKL, TRANCE, tumor necrosis factor ligand superfamily member 11, TNF-related activation-induced cytokine, osteoclast differentiation factor, osteoprotegerin ligand, receptor activator of nuclear factor kappa-B ligand, tumor necrosis factor (ligand) superfamily, member 11, tumor necrosis factor superfamily member 11
  • Host
    Rabbit
  • Protein IDQ9ESE2
  • Protein Name
    Tumor necrosis factor ligand superfamily member 11
  • Scientific Description
    The Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Polyclonal Antibody (Species: Rat) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Rat
  • Reactivity Supplier
    Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RANKL using anti-RANKL antibody (PB10015). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANKL antigen affinity purified polyclonal antibody (Catalog # PB10015) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANKL at approximately 40 kDa. The expected band size for RANKL is at 35 kDa.
Product group Antibodies
Boster Bio
Anti-RANKL/TNFSF11 Antibody Picoband(r)PB10015-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetTnfsf11
  • SizePrice