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ProductsBack/Recoverin antibody [6a55 cd6]
ICC/IF analysis of C6 cells using GTX79211 Recoverin antibody [6a55 cd6]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 oC
ICC/IF analysis of C6 cells using GTX79211 Recoverin antibody [6a55 cd6]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 oC
ICC/IF analysis of C6 cells using GTX79211 Recoverin antibody [6a55 cd6]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 oC

Recoverin antibody [6a55 cd6]

GTX79211
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Rat, Sheep
TargetRCVRN
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Overview

  • Supplier
    GeneTex
  • Product Name
    Recoverin antibody [6a55 cd6]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:100-1:1000. ICC/IF: 1:10-1:200. IHC-P: 1:10-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6a55 cd6
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5957
  • Target name
    RCVRN
  • Target description
    recoverin
  • Target synonyms
    RCV1, recoverin, cancer associated retinopathy antigen, cancer-associated retinopathy protein
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDP35243
  • Protein Name
    Recoverin
  • Scientific Description
    This gene encodes a member of the recoverin family of neuronal calcium sensors. The encoded protein contains three calcium-binding EF-hand domains and may prolong the termination of the phototransduction cascade in the retina by blocking the phosphorylation of photo-activated rhodopsin. Recoverin may be the antigen responsible for cancer-associated retinopathy. [provided by RefSeq, Jul 2008]
  • Reactivity
    Bovine, Human, Rat, Sheep
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Recoverin/RCVRN using anti-Recoverin/RCVRN antibody (A06882-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human ARPE-19 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Recoverin/RCVRN antigen affinity purified polyclonal antibody (Catalog # A06882-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Recoverin/RCVRN at approximately 26kDa. The expected band size for Recoverin/RCVRN is at 23 kDa.
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