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ICC/IF analysis of A431 cells using GTX85210 REEP3 antibody. Dilution : 5 microg/ml
ICC/IF analysis of A431 cells using GTX85210 REEP3 antibody. Dilution : 5 microg/ml
ICC/IF analysis of A431 cells using GTX85210 REEP3 antibody. Dilution : 5 microg/ml

REEP3 antibody

GTX85210
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetREEP3
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Overview

  • Supplier
    GeneTex
  • Product Name
    REEP3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 microg/mL. IHC-P: 5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID221035
  • Target name
    REEP3
  • Target description
    receptor accessory protein 3
  • Target synonyms
    C10orf74; receptor expression-enhancing protein 3; Yip2b
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6NUK4
  • Protein Name
    Receptor expression-enhancing protein 3
  • Scientific Description
    Mammalian odorant receptors require accessory proteins such as RTP1 and RTP2 for functional cell surface expression. Receptor expression-enhancing protein (REEP) family members are transmembrane proteins which interact with odorant receptor proteins and may enhance the odorant receptor responses to odorants. Recently studies have shown other chemosensory receptors such as bitter taste receptors are also influenced by RTP and REEP family members. REEP3 has also been suggested as a novel candidate gene for autism and as a loci influencing the plasma levels of the liver enzyme alkaline phosphatase.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of REEP3 using anti-REEP3 antibody (A12243-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REEP3 antigen affinity purified polyclonal antibody (Catalog # A12243-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REEP3 at approximately 34 kDa. The expected band size for REEP3 is at 29 kDa.
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