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Relaxin 3 antibody [7G14]

GTX52979
GeneTex
ApplicationsWestern Blot, Neutralisation/Blocking
DownloadDatasheet
Product group Antibodies
TargetRLN3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Relaxin 3 antibody [7G14]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    7G14
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID117579
  • Target name
    RLN3
  • Target description
    relaxin 3
  • Target synonyms
    H3; insl7; insulin-like peptide 7; insulin-like peptide INSL7; prorelaxin H3; relaxin-3; RXN3; ZINS4
  • Host
    Mouse
  • Isotype
    IgG2
  • Protein IDQ8WXF3
  • Protein Name
    Relaxin-3
  • Scientific Description
    This gene encodes a member of the relaxin family of insulin-like hormones that is expressed predominantly in the brain and plays a role in physiological processes such as stress, memory and appetite regulation. The encoded protein is a precursor that is proteolytically processed to generate a heterodimeric mature form consisting A and B chains interlinked by disulfide bonds. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RLN3 using anti-RLN3 antibody (A05495-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse tesits tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RLN3 antigen affinity purified polyclonal antibody (Catalog # A05495-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RLN3 at approximately 18-19 kDa. The expected band size for RLN3 is at 15 kDa.
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