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ProductsBack/RELB Antibody (aa219-252)

RELB Antibody (aa219-252)

LS-C408011
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Canine, Guinea Pig, Human, Mammals, Mouse, Rat, Sheep
TargetRELB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RELB Antibody (aa219-252)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (0.1 - 0.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5971
  • Target name
    RELB
  • Target description
    RELB proto-oncogene, NF-kB subunit
  • Target synonyms
    I-REL, IMD53, IREL, REL-B, transcription factor RelB, v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3), v-rel reticuloendotheliosis viral oncogene homolog B, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
  • Host
    Rabbit
  • Reactivity
    Bovine, Canine, Guinea Pig, Human, Mammals, Mouse, Rat, Sheep
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Rel B using anti-Rel B antibody (PB9799). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rel B antigen affinity purified polyclonal antibody (Catalog # PB9799) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rel B at approximately 65-70 kDa. The expected band size for Rel B is at 62 kDa.
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