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ProductsBack/Resistin (RETN) Mouse Monoclonal Antibody
Sandwich ELISA using anti-Human Resistin Antibody Cat.-No AM01373PU-N
Sandwich ELISA using anti-Human Resistin Antibody Cat.-No AM01373PU-N
Sandwich ELISA using anti-Human Resistin Antibody Cat.-No AM01373PU-N

Resistin (RETN) Mouse Monoclonal Antibody

AM01373PU-N
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRETN
Price on request
500 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Resistin (RETN) Mouse Monoclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID56729
  • Target name
    RETN
  • Target description
    resistin
  • Target synonyms
    ADSF, FIZZ3, RENT, RETN1, RSTN, XCP1, resistin, C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1, adipose tissue-specific secretory factor, c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, cysteine-rich secreted protein A12-alpha-like 2, cysteine-rich secreted protein FIZZ3, found in inflammatory zone 3, resistin delta2
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ9HD89
  • Protein Name
    Resistin
  • Scientific Description
    Resistin (RETN) mouse monoclonal antibody, Azide Free
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Resistin using anti-Resistin antibody (PB9412). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human Resistin Protein 0.5ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Resistin antigen affinity purified polyclonal antibody (Catalog # PB9412) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Resistin at approximately 11 kDa. The expected band size for Resistin is at 11 kDa.
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