RFK ELISA Kit (Human)

Aviva Systems Biology

RFK ELISA Kit (Human)

23

Assay Methodology: Quantitative Sandwich Immunoassay

ELISA

0.156-10ng/mL

< 0.27 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Riboflavin Kinase (RFK). Standards or samples are then added to the appropriate...

3h

Research Use Only

Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.

Human

-20°C,2°C to 8°C

41116100

Human