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ProductsBack/RFX1 Antibody
Western Blot Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate All lanes: RFX1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 105 kDa Observed band size: 105 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate All lanes: RFX1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 105 kDa Observed band size: 105 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate All lanes: RFX1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 105 kDa Observed band size: 105 kDa

RFX1 Antibody

CSB-PA019610LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRFX1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RFX1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5989
  • Target name
    RFX1
  • Target description
    regulatory factor X1
  • Target synonyms
    EFC; enhancer factor C; MHC class II regulatory factor RFX; MHC class II regulatory factor RFX1; regulatory factor X, 1 (influences HLA class II expression); RFX; trans-acting regulatory factor 1; transcription factor RFX1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22670
  • Protein Name
    MHC class II regulatory factor RFX1
  • Scientific Description
    Regulatory factor essential for MHC class II genes expression. Binds to the X boxes of MHC class II genes. Also binds to an inverted repeat (ENH1) required for hepatitis B virus genes expression and to the most upstream element (alpha) of the RPL30 promoter.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RFX1 using anti-RFX1 antibody (A04392-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFX1 antigen affinity purified polyclonal antibody (Catalog # A04392-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFX1 at approximately 135 kDa. The expected band size for RFX1 is at 105 kDa.
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