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Western Blot analysis of RAW264.7 cells using RFX3 Polyclonal Antibody
Western Blot analysis of RAW264.7 cells using RFX3 Polyclonal Antibody
Western Blot analysis of RAW264.7 cells using RFX3 Polyclonal Antibody

RFX3 Antibody

CSB-PA003966
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRFX3
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Overview

  • Supplier
    Cusabio
  • Product Name
    RFX3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5991
  • Target name
    RFX3
  • Target description
    regulatory factor X3
  • Target synonyms
    DNA binding protein RFX3; regulatory factor X, 3 (influences HLA class II expression); transcription factor RFX3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48380
  • Protein Name
    Transcription factor RFX3
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RFX3 using anti-RFX3 antibody (A07674-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFX3 antigen affinity purified polyclonal antibody (Catalog # A07674-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFX3 at approximately 95 kDa. The expected band size for RFX3 is at 84 kDa.
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